RNA
Part:BBa_K4830002:Design
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-10)
RNF2 AtoC pegRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design of RNF2 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
Source
The original RNF2 AtoC pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 65777 backbone in our lab.
References
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.